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Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0586)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0586)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0586) at 1:500 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product namePhospho-CD117/c-Kit-Y721 Rabbit pAb
Catalog No.AP0586
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around Y719 of mouse Kit (NP_066922.2).
SequenceTNEYM
Gene ID
Swiss Prot
SynonymsPBT; SCFR; C-Kit; CD117; MASTC; Phospho-CD117/c-Kit-Y721
Calculated MW110kDa
Observed MW120kDa/145kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by EGF
Cellular locationCell membrane, Cytoplasm, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0586)}

Western blot - Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0586)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (AP0586) at 1:500 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)