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ABflo® 488 Rabbit anti-Human CD215/IL-15R alpha mAb (A22582)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD215/IL-15R alpha mAb (A22582)

Flow cytometry:1X10^6 293F cells (negative control, left) and 293F(Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Human CD215/IL-15R mAb(A22582, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product nameABflo® 488 Rabbit anti-Human CD215/IL-15R alpha mAb
Catalog No.A22582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55696-ABf488
This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 31-205 of human CD215/IL-15R alpha (NP_002180.1).
SequenceITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTVTTAGVTPQPESLSPSGKEPAASSPSSNNTAATTAAIVPGSQLMPSKSPSTGTTEISSHESSHGTPSQTTAKNWELTASASHQPPGVYPQGHSDTT
Gene ID
Swiss Prot
SynonymsCD215
Calculated MW28kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples293F CD215 Transfected
Cellular locationCell surface, Endosome, Extracellular space, Nuclear membrane, Plasma membrane

Documents

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD215/IL-15R alpha mAb (A22582)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD215/IL-15R alpha mAb (A22582)

Flow cytometry:1X10^6 293F cells (negative control, left) and 293F(Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Human CD215/IL-15R mAb(A22582, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (4)

재조합 단백질 (4)

Secondary Antibodies (26)