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CD215/IL-15R alpha Rabbit pAb (A2983)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD215/IL-15R alpha Rabbit pAb (A2983)

Western blot analysis of extracts of THP-1 cells, using CD215/IL-15R alpha antibody (A2983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - CD215/IL-15R alpha Rabbit pAb (A2983)

Immunofluorescence analysis of NIH/3T3 cells using CD215/IL-15R alpha Rabbit pAb (A2983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD215/IL-15R alpha Rabbit pAb (A2983)

Immunofluorescence analysis of PC-12 cells using CD215/IL-15R alpha Rabbit pAb (A2983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCD215/IL-15R alpha Rabbit pAb
Catalog No.A2983
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 167-267 of human CD215/IL-15R alpha (NP_002180.1).
SequenceSHESSHGTPSQTTAKNWELTASASHQPPGVYPQGHSDTTVAISTSTVLLCGLSAVSLLACYLKSRQTPPLASVEMEAMEALPVTWGTSSRDEDLENCSHHL
Gene ID
Swiss Prot
SynonymsCD215
Calculated MW28kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesTHP-1
Cellular locationCytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Membrane, Nucleus membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein, extracellular space

Documents

Certificate of Compliance

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ABclonal:Western blot - CD215/IL-15R alpha Rabbit pAb (A2983)}

Western blot - CD215/IL-15R alpha Rabbit pAb (A2983)

Western blot analysis of extracts of THP-1 cells, using CD215/IL-15R alpha antibody (A2983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - CD215/IL-15R alpha Rabbit pAb (A2983)}

Immunofluorescence - CD215/IL-15R alpha Rabbit pAb (A2983)

Immunofluorescence analysis of NIH/3T3 cells using CD215/IL-15R alpha Rabbit pAb (A2983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD215/IL-15R alpha Rabbit pAb (A2983)}

Immunofluorescence - CD215/IL-15R alpha Rabbit pAb (A2983)

Immunofluorescence analysis of PC-12 cells using CD215/IL-15R alpha Rabbit pAb (A2983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

Secondary Antibodies (26)