제품 > 항체 > 단일클론항체(mAb)

CD215/IL-15R alpha Rabbit mAb (A22616)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD215/IL-15R using CD215/IL-15R alpha Rabbit mAb (A22616) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 10s.

ABclonal:Flow CytoMetry - CD215/IL-15R alpha Rabbit mAb (A22616)

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with CD215/IL-15R alpha Rabbit mAb (A22616, 2 μg/mL, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or CD215/IL-15R alpha Rabbit mAb (A22616, 2 μg/mL, right).

Overview

Product name	CD215/IL-15R alpha Rabbit mAb
Catalog No.	A22616
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55696

Background

This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.

Immunogen information

Immunogen	Recombinant protein of human CD215/IL-15R alpha.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	CD215; CD215/IL-15R alpha
Calculated MW	28kDa
Observed MW	40-55kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	293F
Cellular location	Cytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Membrane, Nucleus membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein, extracellular space.