제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-Human CD215/IL-15R alpha mAb (A23849)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293F cells(negative control, left) and 293F(Transfection, right) cells were surface-stained with ABflo® 594 Rabbit anti-Human CD215/IL-15R alpha mAb(A23849, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293T(Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD215/IL-15R alpha mAb(A23849, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Human CD215/IL-15R alpha mAb
Catalog No.	A23849
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55696-ABf594

Background

This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 31-205 of human CD215/IL-15R alpha（NP_002180.1）.
Sequence	ITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTVTTAGVTPQPESLSPSGKEPAASSPSSNNTAATTAAIVPGSQLMPSKSPSTGTTEISSHESSHGTPSQTTAKNWELTASASHQPPGVYPQGHSDTT
Gene ID
Swiss Prot
Synonyms	IL15RA; CD215; interleukin-15 receptor subunit alpha
Calculated MW	15-28kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Membrane, Nucleus membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein, extracellular space