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ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)

Flow cytometry: 1X10^6 CHO (Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD9 mAb (A24158, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)

Flow cytometry: 1X10^6 CHO cells (negative control, left) and CHO (Transfection, right) cells were surface-stained with ABflo® 594 Rabbit anti-Human CD9 mAb (A24158, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)

Flow cytometry:1X10^6 Human PBMC were surface-stained with PE-Cy7 Mouse anti-Human CD3 mAb (A22798, 5 μl/Test) and ABflo® 594 Rabbit anti-Human CD9 mAb (A24158, 5 μl/Test, right) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left).

Overview

Product nameABflo® 594 Rabbit anti-Human CD9 mAb
Catalog No.A24158
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65639-ABflo594
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human CD9(NP_001760.1).
SequenceMPVKGGTKCIKYLLFGFNFIFWLAGIAVLAIGLWLRFDSQTKSIFEQETNNNNSSFYTGVYILIGAGALMMLVGFLGCCGAVQESQCMLGLFFGFLLVIFAIEIAAAIWGYSHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV
Gene ID
Swiss Prot
SynonymsMIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Calculated MW25kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Membrane, Multi-pass membrane protein.

Documents

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ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)

Flow cytometry: 1X10^6 CHO (Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD9 mAb (A24158, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)

Flow cytometry: 1X10^6 CHO cells (negative control, left) and CHO (Transfection, right) cells were surface-stained with ABflo® 594 Rabbit anti-Human CD9 mAb (A24158, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD9 mAb (A24158)

Flow cytometry:1X10^6 Human PBMC were surface-stained with PE-Cy7 Mouse anti-Human CD3 mAb (A22798, 5 μl/Test) and ABflo® 594 Rabbit anti-Human CD9 mAb (A24158, 5 μl/Test, right) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left).

* For research use only. Not for therapeutic or diagnostic purposes.

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