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ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311, 5 μl/Test, orange line) or ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, left) or ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311, 5 μl/Test, right).

Overview

Product nameABflo® 610 Rabbit anti-Mouse CD48 mAb
Catalog No.A27311
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67324-ABflo610
Predicted to enable antigen binding activity and signaling receptor activity. Acts upstream of or within T cell activation and signal transduction. Located in external side of plasma membrane. Is expressed in left lung; liver; right lung; and thymus primordium. Orthologous to human CD48 (CD48 molecule).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-216 of mouse CD48 (NP_031675.1).
SequenceFQGHSIPDINATTGSNVTLKIHKDPLGPYKRITWLHTKNQKILEYNYNSTKTIFESEFKGRVYLEENNGALHISNVRKEDKGTYYMRVLRETENELKITLEVFDPVPKPSIEINKTEASTDSCHLRLSCEVKDQHVDYTWYESSGPFPKKSPGYVLDLIVTPQNKSTFYTCQVSNPVSSKNDTVYFTLPCDLAR
Gene ID
Swiss Prot
SynonymsBCM1; BLAST; Bcm-1; BLAST1; SLAMF2; Sgp-60; BLAST-1; MEM-102
Calculated MW27kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Secreted.

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    ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311)}

    Flow CytoMetry - ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311)

    Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311, 5 μl/Test, orange line) or ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311)}

    Flow CytoMetry - ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, left) or ABflo® 610 Rabbit anti-Mouse CD48 mAb (A27311, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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