제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767, 5 μl/Test, right).

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and Mouse PBMC (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Mouse PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767, 5 μl/Test, right).

Flow cytometry:1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 488 Rabbit anti-Mouse CD34 mAb (A23107, 5 μl/Test) and ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767, 5 μl/Test, left) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, right).

Flow cytometry:1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 488 Rabbit anti-Mouse CD2 mAb (A23704, 5 μl/Test) and ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767, 5 μl/Test, left) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Mouse CD48 mAb
Catalog No.	A25767
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC67324-ABflo647

Background

Predicted to enable antigen binding activity and signaling receptor activity. Acts upstream of or within T cell activation and signal transduction. Located in external side of plasma membrane. Is expressed in left lung; liver; right lung; and thymus primordium. Orthologous to human CD48 (CD48 molecule).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 23-216 of mouse CD48 (NP_031675.1).
Sequence	FQGHSIPDINATTGSNVTLKIHKDPLGPYKRITWLHTKNQKILEYNYNSTKTIFESEFKGRVYLEENNGALHISNVRKEDKGTYYMRVLRETENELKITLEVFDPVPKPSIEINKTEASTDSCHLRLSCEVKDQHVDYTWYESSGPFPKKSPGYVLDLIVTPQNKSTFYTCQVSNPVSSKNDTVYFTLPCDLAR
Gene ID
Swiss Prot
Synonyms	BCM1; BLAST; Bcm-1; BLAST1; SLAMF2; Sgp-60; BLAST-1; MEM-102
Calculated MW	27kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Secreted

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

ABflo® 647 Rabbit anti-Mouse CD48 mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767)

Flow cytometry: 1X10^6 Mouse PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767, 5 μl/Test, right).

Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD48 mAb (A25767)

* For research use only. Not for therapeutic or diagnostic purposes.