제품 > 항체 > 단일클론항체(mAb)

CD48 Rabbit mAb (A26044)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse splenocytes (right) were surface-stained with CD48 Rabbit mAb (A26044, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD48 Rabbit mAb (A26044, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

Overview

Product name	CD48 Rabbit mAb
Catalog No.	A26044
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC67324

Background

Predicted to enable antigen binding activity and signaling receptor activity. Acts upstream of or within T cell activation and signal transduction. Located in external side of plasma membrane. Is expressed in left lung; liver; right lung; and thymus primordium. Orthologous to human CD48 (CD48 molecule).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 23-216 of mouse CD48 (NP_031675.1).
Sequence	FQGHSIPDINATTGSNVTLKIHKDPLGPYKRITWLHTKNQKILEYNYNSTKTIFESEFKGRVYLEENNGALHISNVRKEDKGTYYMRVLRETENELKITLEVFDPVPKPSIEINKTEASTDSCHLRLSCEVKDQHVDYTWYESSGPFPKKSPGYVLDLIVTPQNKSTFYTCQVSNPVSSKNDTVYFTLPCDLAR
Gene ID
Swiss Prot
Synonyms	BCM1; BLAST; Bcm-1; BLAST1; SLAMF2; Sgp-60; BLAST-1; MEM-102
Calculated MW	27kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Secreted