제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)

Confocal imaging of 293T cells transfected with CD252 (OX40 Ligand) using ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746, dilution 1:100) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb
Catalog No.A25746
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66508-ABflo647
Enables tumor necrosis factor receptor binding activity. Involved in several processes, including T cell activation; regulation of gene expression; and regulation of lymphocyte activation. Acts upstream of or within cholesterol metabolic process; inflammatory response; and negative regulation of cytokine production. Predicted to be located in cell surface. Predicted to be active in extracellular space. Is expressed in sensory organ and skeleton. Used to study primary pulmonary hypertension and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in myocardial infarction. Orthologous to human TNFSF4 (TNF superfamily member 4).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 51-198 of mouse CD252 (OX40 Ligand) (NP_033478.1).
SequenceSSSPAKDPPIQRLRGAVTRCEDGQLFISSYKNEYQTMEVQNNSVVIKCDGLYIIYLKGSFFQEVKIDLHFREDHNPISIPMLNDGRRIVFTVVASLAFKDKVYLTVNAPDTLCEHLQINDGELIVVQLTPGYCAPEGSYHSTVNQVPL
Gene ID
Swiss Prot
SynonymsAth1; gp34; Ath-1; Ox40l; TXGP1; CD134L; OX-40L; Tnlg2b; Txgp1l
Calculated MW22kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type II membrane protein.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)}

    Immunofluorescence - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)

    Confocal imaging of 293T cells transfected with CD252 (OX40 Ligand) using ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746, dilution 1:100) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)

    Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746)

    Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD252 (OX40 Ligand) mAb (A25746, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

    재조합 단백질 (1)

    ELISA 키트 (1)

    항체 (3)

    Secondary Antibodies (26)