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ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236)

Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and U-87MG cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236)

Flow cytometry: 1X10^6 U-87MG cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236, 5 μl/Test, right).

Overview

Product nameABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb
Catalog No.A26236
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59957-ABflo700
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 33-530 of human CD140b/PDGFRβ(NP_002600.1).
SequenceLVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPF
Gene ID
Swiss Prot
SynonymsPDGFRB; CD140B; IBGC4; IMF1; JTK12; KOGS; PDGFR; PDGFR-1; PDGFR1; PENTT; platelet-derived growth factor receptor beta
Calculated MW37kDa/123kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein.

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ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236)}

Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236)

Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and U-87MG cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236)}

Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236)

Flow cytometry: 1X10^6 U-87MG cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human CD140b/PDGFRβ mAb (A26236, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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