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Phospho-PDGFRB-Y740 Rabbit pAb (AP0556)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Phospho-PDGFRB-Y740 Rabbit pAb (AP0556)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y740 antibody (AP0556).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-PDGFRB-Y740 Rabbit pAb
Catalog No.AP0556
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.
ImmunogenA synthetic phosphorylated peptide around Y740 of human PDGFRB (NP_002600.1).
SequenceGGYMD
Gene ID
Swiss Prot
SynonymsIMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
Calculated MW124kDa
Observed MW180kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3
Cellular locationCell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - Phospho-PDGFRB-Y740 Rabbit pAb (AP0556)}

Western blot - Phospho-PDGFRB-Y740 Rabbit pAb (AP0556)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y740 antibody (AP0556).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (9)

재조합 단백질 (2)

Secondary Antibodies (26)