제품 > 항체 > 단일클론항체(mAb)

CD140b/PDGFRB Rabbit mAb (A23952)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using CD140b/PDGFRB Rabbit mAb (A23952) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Flow CytoMetry - CD140b/PDGFRB Rabbit mAb (A23952)

Flow cytometry:1X10^6 MCF7 cells (negative control, left) and U-87MG (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD140b/PDGFRB mAb(A23952, 2 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 U-87MG cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, left) or CD140b/PDGFRB Rabbit mAb(A23952, 2 μg/mL, right).

Overview

Product name	CD140b/PDGFRB Rabbit mAb
Catalog No.	A23952
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59959

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 33-530 of human CD140b/PDGFRB(NP_002600.1).
Sequence	LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPF
Gene ID
Swiss Prot
Synonyms	PDGFRB; CD140B; IBGC4; IMF1; JTK12; KOGS; PDGFR; PDGFR-1; PDGFR1; PENTT; platelet-derived growth factor receptor beta; CD140b/PDGFRB
Calculated MW	37kDa/123kDa
Observed MW	190kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:8000 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	SH-SY5Y, U-87MG
Cellular location	Cell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Research Area

CD140b/PDGFRB Rabbit mAb images

Western blot - CD140b/PDGFRB Rabbit mAb (A23952)

Flow CytoMetry - CD140b/PDGFRB Rabbit mAb (A23952)

Flow cytometry:1X10^6 U-87MG cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, left) or CD140b/PDGFRB Rabbit mAb(A23952, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.