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PDGFRB Rabbit pAb (A1444)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PDGFRB Rabbit pAb (A1444)

Western blot analysis of various lysates using PDGFRB Rabbit pAb (A1444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - PDGFRB Rabbit pAb (A1444)

Western blot analysis of lysates from SH-SY5Y cells, using PDGFRB Rabbit pAb (A1444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PDGFRB Rabbit pAb (A1444)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using PDGFRB Rabbit pAb (A1444) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PDGFRB Rabbit pAb (A1444)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using PDGFRB Rabbit pAb (A1444) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PDGFRB Rabbit pAb (A1444)

Immunofluorescence analysis of NIH-3T3 cells using PDGFRB Rabbit pAb (A1444) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePDGFRB Rabbit pAb
Catalog No.A1444
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 900-1000 of human PDGFRB (NP_002600.1).
SequenceGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRS
Gene ID
Swiss Prot
SynonymsIMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1; PDGFRB
Calculated MW124kDa
Observed MW190kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, NIH/3T3, C6
Cellular locationCell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

IHC(Mus musculus)

mIHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - PDGFRB Rabbit pAb (A1444)}

Western blot - PDGFRB Rabbit pAb (A1444)

Western blot analysis of various lysates using PDGFRB Rabbit pAb (A1444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - PDGFRB Rabbit pAb (A1444)}

Western blot - PDGFRB Rabbit pAb (A1444)

Western blot analysis of lysates from SH-SY5Y cells, using PDGFRB Rabbit pAb (A1444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - PDGFRB Rabbit pAb (A1444)}

Immunohistochemistry - PDGFRB Rabbit pAb (A1444)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using PDGFRB Rabbit pAb (A1444) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PDGFRB Rabbit pAb (A1444)}

Immunohistochemistry - PDGFRB Rabbit pAb (A1444)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using PDGFRB Rabbit pAb (A1444) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PDGFRB Rabbit pAb (A1444)}

Immunofluorescence - PDGFRB Rabbit pAb (A1444)

Immunofluorescence analysis of NIH-3T3 cells using PDGFRB Rabbit pAb (A1444) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1444? Please let us know so that we can cite the reference in this datasheet.

항체 (9)

재조합 단백질 (2)

Secondary Antibodies (26)