제품 > 항체 > 다중클론항체(pAb)

AKT2 Rabbit pAb (A18019)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - AKT2 Rabbit pAb (A18019)

Western blot analysis of various lysates using AKT2 Rabbit pAb (A18019) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - AKT2 Rabbit pAb (A18019)

Western blot analysis of lysates from 293T cells, using AKT2 Rabbit pAb (A18019) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameAKT2 Rabbit pAb
Catalog No.A18019
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 381-481 of human AKT2 (NP_001617.1).
SequenceLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIRE
Gene ID
Swiss Prot
SynonymsPKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; AKT2
Calculated MW56kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Raji, Jurkat, HeLa, LO2, Mouse kidney
Cellular locationCell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - AKT2 Rabbit pAb (A18019)}

Western blot - AKT2 Rabbit pAb (A18019)

Western blot analysis of various lysates using AKT2 Rabbit pAb (A18019) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - AKT2 Rabbit pAb (A18019)}

Western blot - AKT2 Rabbit pAb (A18019)

Western blot analysis of lysates from 293T cells, using AKT2 Rabbit pAb (A18019) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18019? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)