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Phospho-AKT2 specific-S474 Rabbit pAb (AP1403)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (AP1403) at 1:900 dilution. 293F cells were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from 293F cells using Phospho-AKT2 specific-S474 Rabbit pAb (AP1403) at 1:900 dilution. 293F cells, 293F cells transfected with AKT3 and 293F cells transfected with AKT2 were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	Phospho-AKT2 specific-S474 Rabbit pAb
Catalog No.	AP1403
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S474 of human AKT2 specific .
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; Phospho-AKT2 specific-S474
Calculated MW	56kDa
Observed MW	63kDa/90kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293F treated by Calyculin A, 293F treated by Calyculin A after transfected with AKT2
Cellular location	Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein.
Customer validation	WB(Mus musculus)