제품 > 항체 > 다중클론항체(pAb)

Ambra1 Rabbit pAb (A1083)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from PANC-1 cells, using Ambra1 Rabbit pAb (A1083) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Ambra1 Rabbit pAb (A1083)

Immunohistochemistry analysis of paraffin-embedded rat lung using Ambra1 Rabbit pAb (A1083) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse lung using Ambra1 Rabbit pAb (A1083) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Ambra1 Rabbit pAb (A1083)

Immunofluorescence analysis of NIH/3T3 cells using Ambra1 Rabbit pAb (A1083) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Ambra1 Rabbit pAb
Catalog No.	A1083
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables GTPase binding activity and ubiquitin protein ligase binding activity. Involved in macroautophagy; positive regulation of phosphatidylinositol 3-kinase activity; and response to mitochondrial depolarisation. Located in cytosol. Colocalizes with mitochondrion. Biomarker of multiple system atrophy.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1208 of human Ambra1 (NP_060219.2).
Sequence	GAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR
Gene ID
Swiss Prot
Synonyms	DCAF3; WDR94; Ambra1
Calculated MW	143kDa
Observed MW	130-150kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	PANC-1
Cellular location	Cytoplasmic vesicle, autophagosome
Customer validation	IF(Homo sapiens)