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BRD4 Rabbit pAb (A18840)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BRD4 Rabbit pAb (A18840)

Western blot analysis of lysates from HeLa cells, using BRD4 Rabbit pAb (A18840) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - BRD4 Rabbit pAb (A18840)

Immunohistochemistry analysis of paraffin-embedded rat testis using BRD4 Rabbit pAb (A18840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BRD4 Rabbit pAb (A18840)

Immunohistochemistry analysis of paraffin-embedded human stomach using BRD4 Rabbit pAb (A18840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameBRD4 Rabbit pAb
Catalog No.A18840
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700-794 of human BRD4 (NP_001317313.1).
SequenceSSESESSSESSSSDSEDSETAFCTSGDFVSPGPSPYHSHVQCGRFREMLRWFLVDVEQTAAGQPHRQSAAGPAITWAPAIAYPSPECARCCVGCS
Gene ID
Swiss Prot
SynonymsCAP; MCAP; HUNK1; HUNKI; FSHRG4; BRD4
Calculated MW152kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HeLa-BRD4-FL(Flag-tag), HeLa-BRD4-S(a)(Flag-tag), HeLa-BRD4-S(b)(Flag-tag)
Cellular locationChromosome, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - BRD4 Rabbit pAb (A18840)}

Western blot - BRD4 Rabbit pAb (A18840)

Western blot analysis of lysates from HeLa cells, using BRD4 Rabbit pAb (A18840) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - BRD4 Rabbit pAb (A18840)}

Immunohistochemistry - BRD4 Rabbit pAb (A18840)

Immunohistochemistry analysis of paraffin-embedded rat testis using BRD4 Rabbit pAb (A18840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BRD4 Rabbit pAb (A18840)}

Immunohistochemistry - BRD4 Rabbit pAb (A18840)

Immunohistochemistry analysis of paraffin-embedded human stomach using BRD4 Rabbit pAb (A18840) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (10)

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