Phospho-BRD4-S484/S488 Rabbit pAb (AP1177)

Publication (2) Datasheet

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

ABclonal:Dot Blot - Phospho-BRD4-S484/S488 Rabbit pAb (AP1177)

Dot-blot analysis of all sorts of peptides using Phospho-BRD4-S484/S488 antibody (AP1177) at 1:1000 dilution.

Overview

Product name	Phospho-BRD4-S484/S488 Rabbit pAb
Catalog No.	AP1177
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S484 & S488 of human BRD4 (NP_490597.1).
Sequence	SSSDS
Gene ID
Swiss Prot
Synonyms	CAP; MCAP; HUNK1; HUNKI; FSHRG4; Phospho-BRD4-S484/S488
Calculated MW	152kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 DB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples
Cellular location	Chromosome, Nucleus.
Customer validation	IF(Homo sapiens) WB(Homo sapiens)