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Phospho-BRD4-S1083 Rabbit mAb (AP1235)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates using Phospho-BRD4-S1083 Rabbit mAb (AP1235) at1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 300s.

Overview

Product name	Phospho-BRD4-S1083 Rabbit mAb
Catalog No.	AP1235
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5019-01

Background

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S1083 of human BRD4 (NP_490597.1).
Sequence	HQSPP
Gene ID
Swiss Prot
Synonyms	CAP; MCAP; HUNK1; HUNKI; FSHRG4; Phospho-BRD4-S1083
Calculated MW	152kDa
Observed MW	200kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Calyculin A after transfected with BRD4 (Full length), HeLa treated by nocodazole after transfected with BRD4 (Full length)
Cellular location	condensed nuclear chromosome, nucleoplasm, nucleus.