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Phospho-BRD4-S498/S499 Rabbit pAb (AP1179)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-BRD4-S498/S499 Rabbit pAb (AP1179)

Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S498/S499 Rabbit pAb (AP1179) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product namePhospho-BRD4-S498/S499 Rabbit pAb
Catalog No.AP1179
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
ImmunogenA synthetic phosphorylated peptide around S498 & S499 of human BRD4 (NP_490597.1).
SequenceSDSSTD
Gene ID
Swiss Prot
SynonymsCAP; MCAP; HUNK1; HUNKI; FSHRG4; Phospho-BRD4-S498/S499
Calculated MW152kDa
Observed MW125kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by Calyculin A
Cellular locationChromosome, Nucleus.
ABclonal:Western blot - Phospho-BRD4-S498/S499 Rabbit pAb (AP1179)}

Western blot - Phospho-BRD4-S498/S499 Rabbit pAb (AP1179)

Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S498/S499 Rabbit pAb (AP1179) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)