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CD44 Rabbit mAb (A21919)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD44 Rabbit mAb (A21919)

Western blot analysis of lysates from U-251MG cells, using CD44 Rabbit mAb (A21919) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD44 Rabbit mAb (A21919)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD44 Rabbit mAb (A21919) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD44 Rabbit mAb (A21919)

Immunohistochemistry analysis of paraffin-embedded Human skin using CD44 Rabbit mAb (A21919) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameCD44 Rabbit mAb
Catalog No.A21919
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0464
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human CD44 (P16070).
SequenceNNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFST
Gene ID
Swiss Prot
SynonymsIN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; H-CAM; HCELL; ECM-III; HUTCH-1; HUTCH-I; ECMR-III; Hermes-1; CD44
Calculated MW82kDa
Observed MW80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:500 - 1:2000
  • FC 1:20 - 1:50
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Flow Cytometry    
Positive samplesU-251 MG
Cellular locationCell membrane, Single-pass type I membrane protein.
Customer validation

IF(Mus musculus)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD44 Rabbit mAb (A21919)}

Western blot - CD44 Rabbit mAb (A21919)

Western blot analysis of lysates from U-251MG cells, using CD44 Rabbit mAb (A21919) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CD44 Rabbit mAb (A21919)}

Immunohistochemistry - CD44 Rabbit mAb (A21919)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD44 Rabbit mAb (A21919) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD44 Rabbit mAb (A21919)}

Immunohistochemistry - CD44 Rabbit mAb (A21919)

Immunohistochemistry analysis of paraffin-embedded Human skin using CD44 Rabbit mAb (A21919) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A21919? Please let us know so that we can cite the reference in this datasheet.

항체 (7)

재조합 단백질 (3)

ELISA 키트 (1)

Secondary Antibodies (24)