제품 > 항체 > 단일클론항체(mAb)

Caspase-3 Rabbit mAb (A25309)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells using Caspase-3 Rabbit mAb (A25309) at 1:5000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated by staurosporine(1 uM) for 3 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Caspase-3 Rabbit mAb (A25309)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Caspase-3 Rabbit mAb (A25309) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Caspase-3 Rabbit mAb (A25309) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	Caspase-3 Rabbit mAb
Catalog No.	A25309
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC3244

Background

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Caspase-3 (NP_004337.2).
Sequence	INNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRS
Gene ID
Swiss Prot
Synonyms	CPP32; SCA-1; CPP32B; Caspase-3
Calculated MW	32kDa
Observed MW	32kDa/17, 19kDa/17, 19kDa, 35kDa/17/19/35kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:100 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HAP1, HeLa, HeLa
Cellular location	Cytoplasm.
Customer validation	WB(Mus musculus, Homo sapiens, Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Caspase-3 Rabbit mAb images

Western blot - Caspase-3 Rabbit mAb (A25309)

Immunohistochemistry - Caspase-3 Rabbit mAb (A25309)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Molecular neurobiology

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38087170

Title:

Conditional Knockout of IL-1R1 in Endothelial Cells Attenuates Seizures and Neurodegeneration via Inhibiting Neuroinflammation Mediated by Nrf2/HO-1

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Microorganisms

Application:

IF:

4.5

Species:

Homo sapiens

PMID:

38276212

Title:

miR-4687-5p Affects Intracellular Survival of Mycobacterium tuberculosis through Its Regulation of NRAMP1 Expression in A549 Cells - PMC

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Journal of enzyme inhibition and medicinal chemistry

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

38086769

Title:

Novel isobavachalcone derivatives induce apoptosis and necroptosis in human non-small cell lung cancer H1975 cells

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Advanced healthcare materials

Application:

IF:

Species:

Mus musculus

PMID:

38551143

Title:

Engineered Luminescent Oncolytic Vaccinia Virus Activation of PhotodynamicImmune Combination Therapy for Colorectal Cancer

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Biomacromolecules

Application:

IF:

5.5

Species:

Mus musculus

PMID:

38779908

Title:

Paclitaxel Overload Supramolecular Oxidative Stress Nanoamplifier with a CDK12 Inhibitor for Enhanced Cancer Therapy

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

38944012

Title:

The combined effects of polystyrene of different sizes and cadmium in mouse kidney tissues

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.7

Species:

Gallus gallus

PMID:

39199181

Title:

Pterostilbene, a Resveratrol Derivative, Improves Ovary Function by Upregulating Antioxidant Defenses in the Aging Chickens via Increased SIRT1/Nrf2 Expression - PMC

References for Caspase-3 Rabbit mAb (A25309)

Product:

Caspase-3 Rabbit mAb

Journal:

Actas espanolas de psiquiatria

Application:

IF:

Species:

Homo sapiens

PMID:

39129701

Title: