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FGFR2 Rabbit pAb (A12436)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FGFR2 Rabbit pAb (A12436)

Western blot analysis of extracts of MCF7 cells, using FGFR2 antibody (A12436) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - FGFR2 Rabbit pAb (A12436)

Immunofluorescence analysis of HeLa cells using FGFR2 antibody (A12436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FGFR2 Rabbit pAb (A12436)

Immunofluorescence analysis of NIH/3T3 cells using FGFR2 antibody (A12436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FGFR2 Rabbit pAb (A12436)

Immunofluorescence analysis of PC-12 cells using FGFR2 antibody (A12436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameFGFR2 Rabbit pAb
Catalog No.A12436
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 360-462 of human FGFR2 (NP_000132.3).
SequenceLPAPGREKEITASPDYLEIAIYCIGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMNSNTPLVRITTRLSSTADTPMLAG
Gene ID
Swiss Prot
SynonymsBEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Calculated MW92kDa
Observed MW145kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7
Cellular locationCell membrane, Cytoplasmic vesicle, Golgi apparatus, Secreted, Single-pass type I membrane protein
Customer validation

WB(Rattus norvegicus, Mus musculus )

Documents

Certificate of Compliance

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ABclonal:Western blot - FGFR2 Rabbit pAb (A12436)}

Western blot - FGFR2 Rabbit pAb (A12436)

Western blot analysis of extracts of MCF7 cells, using FGFR2 antibody (A12436) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - FGFR2 Rabbit pAb (A12436)}

Immunofluorescence - FGFR2 Rabbit pAb (A12436)

Immunofluorescence analysis of HeLa cells using FGFR2 antibody (A12436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FGFR2 Rabbit pAb (A12436)}

Immunofluorescence - FGFR2 Rabbit pAb (A12436)

Immunofluorescence analysis of NIH/3T3 cells using FGFR2 antibody (A12436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FGFR2 Rabbit pAb (A12436)}

Immunofluorescence - FGFR2 Rabbit pAb (A12436)

Immunofluorescence analysis of PC-12 cells using FGFR2 antibody (A12436) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12436? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

재조합 단백질 (8)

Secondary Antibodies (26)