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GAPDH Rabbit mAb(High Dilution) (A19056)

Publications (361) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GAPDH Rabbit mAb(High Dilution) (A19056)

Western blot analysis of various lysates, using GAPDH Rabbit mAb (A19056) at 1:100000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - GAPDH Rabbit mAb(High Dilution) (A19056)

Western blot analysis of lysates from HeLa cells, using GAPDH Rabbit mAb (A19056) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Human colon using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Human spleen using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Rat colon using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GAPDH Rabbit mAb(High Dilution) (A19056)

Confocal imaging of HeLa cells using GAPDH Rabbit mAb (A19056, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameGAPDH Rabbit mAb(High Dilution)
Catalog No.A19056
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC50888
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants..
Note：Due to the high antibody titer, it is advisable to be diluted before use. Please dilute 5μL of the antibody solution with 45μL of PBS solution, containing 50% glycerol. The diluted antibody can be stored at -20°C.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 4-335 of human GAPDH (NP_002037.2).
SequenceVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID
Swiss Prot
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:50000 - 1:200000
  • IHC-P 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, HepG2, Mouse liver, Mouse kidney, Mouse spleen, Rat liver
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region.
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Ictalurus punctatus, Cordyceps militaris, Sus scrofa, Oryctolagus cuniculus, Acidovorax citrulli, Rattus norvegicus, Chlorocebus aethiops, Mus musculus, Danio rerio, Ovis aries, Other, Bos taurus, Canis familiaris, Bos taurus, Arabidopsis thaliana)

IP(Homo sapiens, Mus musculus)

IP(Homo sapiens, Other)

IF(Mus musculus, Sus scrofa)

ELISA(Mus musculus)

WB(Mus musculus)

IHC(Mus musculus)

WB(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - GAPDH Rabbit mAb(High Dilution) (A19056)}

Western blot - GAPDH Rabbit mAb(High Dilution) (A19056)

Western blot analysis of various lysates, using GAPDH Rabbit mAb (A19056) at 1:100000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - GAPDH Rabbit mAb(High Dilution) (A19056)}

Western blot - GAPDH Rabbit mAb(High Dilution) (A19056)

Western blot analysis of lysates from HeLa cells, using GAPDH Rabbit mAb (A19056) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)}

Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Human colon using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)}

Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Human spleen using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)}

Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)}

Immunohistochemistry - GAPDH Rabbit mAb(High Dilution) (A19056)

Immunohistochemistry analysis of paraffin-embedded Rat colon using GAPDH Rabbit mAb (A19056) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - GAPDH Rabbit mAb(High Dilution) (A19056)}

Immunofluorescence - GAPDH Rabbit mAb(High Dilution) (A19056)

Confocal imaging of HeLa cells using GAPDH Rabbit mAb (A19056, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19056? Please let us know so that we can cite the reference in this datasheet.

항체 (6)

GAPDH Mouse mAb
AC002

ELISAWBIHC-PIF/ICC

Reactivity:Human, Mouse, Rat

GAPDH Mouse mAb

GAPDH Mouse mAb (High Dilution)
AC033

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

GAPDH Mouse mAb (High Dilution)

GAPDH Rabbit pAb
AC027

WBIHC-PIF/ICCIP

Reactivity:Human, Mouse, Rat

GAPDH Rabbit pAb

GAPDH (Human Specific) Rabbit mAb
AC036

ELISAWBIF/ICC

Reactivity:Human

GAPDH (Human Specific) Rabbit mAb

HRP-conjugated GAPDH Mouse mAb
AC035

ELISAWB

Reactivity:Human, Mouse, Rat

HRP-conjugated GAPDH Mouse mAb

HRP-conjugated GAPDH Rabbit mAb
AC054

ELISAWB

Reactivity:Human, Mouse, Rat

HRP-conjugated GAPDH Rabbit mAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)