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GLI1 Rabbit mAb (A23236)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GLI1 Rabbit mAb (A23236)

Western blot analysis of lysates from A-549 cells using GLI1 Rabbit mAb (A23236) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GLI1 Rabbit mAb (A23236)

Immunofluorescence analysis of Neuro-2a cells using GLI1 Rabbit mAb (A23236) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGLI1 Rabbit mAb
Catalog No.A23236
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59108
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human GLI1 (NP_005260.1).
SequenceMFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVY
Gene ID
Swiss Prot
SynonymsGLI; PPD1; PAPA8; GLI1
Calculated MW118kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - GLI1 Rabbit mAb (A23236)}

Western blot - GLI1 Rabbit mAb (A23236)

Western blot analysis of lysates from A-549 cells using GLI1 Rabbit mAb (A23236) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)}

Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)}

Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)}

Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GLI1 Rabbit mAb (A23236)}

Immunohistochemistry - GLI1 Rabbit mAb (A23236)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using GLI1 Rabbit mAb (A23236) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - GLI1 Rabbit mAb (A23236)}

Immunofluorescence - GLI1 Rabbit mAb (A23236)

Immunofluorescence analysis of Neuro-2a cells using GLI1 Rabbit mAb (A23236) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)