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HRP-conjugated β-Actin Mouse mAb (AC043)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using HRP-conjugated β-Actin Mouse mAb (AC043) at 1:5000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb (AC043) at 1:2000- 1:640000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	HRP-conjugated β-Actin Mouse mAb
Catalog No.	AC043
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1, Lambda
CloneNo.	AMC0001

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.

Immunogen information

Immunogen	Recombinant protein of Mouse HRP-conjugated β-Actin
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	BRWS1; PS1TP5BP1; HRP-conjugated β-Actin
Calculated MW	42kDa
Observed MW	42kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:50000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T, HeLa, A-549, NIH/3T3, Mouse lung, Mouse brain, Rat testis, Rat spleen
Cellular location	Cytoplasm, cytoskeleton.
Customer validation	WB(Ctenopharyngodon idellus, Rattus norvegicus, Homo sapiens, Mus musculus)