Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (A8086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using DDIT4 antibody (A8086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
| Product name | [KO Validated] DDIT4 Rabbit pAb |
|---|---|
| Catalog No. | A8086 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1). |
|---|---|
| Sequence | MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Dig2; REDD1; REDD-1 |
| Calculated MW | 25kDa |
| Observed MW | 32kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | A-549, DU145, U-251MG |
| Cellular location | Cytoplasm, Mitochondrion, cytosol |
| Customer validation | (SH-SY5Y) IHC(Homo sapiens) WB(Homo sapiens, Mus musculus) |
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![ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)](https://abclonal-us.oss-us-east-1.aliyuncs.com/abclonal/Catalog/A8086/A8086_1.jpg?t=1690982788 "ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)")
![ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)](https://abclonal-us.oss-us-east-1.aliyuncs.com/abclonal/Catalog/A8086/A8086_2.jpg?t=1690982799 "ABclonal:Western blot - [KO Validated] DDIT4 Rabbit pAb (A8086)")
![ABclonal: review for [KO Validated] DDIT4 Rabbit pAb(A8086)](https://abclonal-us.oss-us-east-1.aliyuncs.com/abclonal/ReviewImages/A8086_20240626144905_FB0002444.png?t=1719384547 "ABclonal: review for [KO Validated] DDIT4 Rabbit pAb(A8086)")
