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Phospho-YAP1-S127 Rabbit pAb (AP0489)

Review (1)Publications (33) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-YAP1-S127 Rabbit pAb (AP0489) at 1:400 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunofluorescence analysis of C6 cells (treated with CalycμLin A) and C6 cells (untreated) using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-YAP1-S127 Rabbit pAb(AP0489)

Overview

Product namePhospho-YAP1-S127 Rabbit pAb
Catalog No.AP0489
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1).
SequenceAHSSP
Gene ID
Swiss Prot
SynonymsYAP; YKI; COB1; YAP2; YAP-1; YAP65; Phospho-YAP1-S127
Calculated MW54kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3 treated by Calyculin A
Cellular locationCytoplasm, Nucleus
Customer validation

(THP1)

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Xenopus laevis)

IF(Danio rerio, Homo sapiens)

IP(Homo sapiens)

IF(Homo sapiens)

IHC(Homo sapiens, Mus musculus)

WB(Homo sapiens)

Co-IP(Homo sapiens)

IF(Homo sapiens)

WB (Mus musculus )

ChIP-Seq(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Phospho-YAP1-S127 Rabbit pAb (AP0489)}

Western blot - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-YAP1-S127 Rabbit pAb (AP0489) at 1:400 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)}

Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)}

Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)}

Immunohistochemistry - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:25 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-YAP1-S127 Rabbit pAb (AP0489)}

Immunofluorescence - Phospho-YAP1-S127 Rabbit pAb (AP0489)

Immunofluorescence analysis of C6 cells (treated with CalycμLin A) and C6 cells (untreated) using Phospho-YAP1-S127 Rabbit pAb (AP0489) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (9)

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MonoMethyl-YAP1-K342 Rabbit pAb
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ELISAWB

Reactivity:Human, Mouse, Rat

MonoMethyl-YAP1-K342 Rabbit pAb

Non-phospho-YAP1-S127 Rabbit pAb
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WBIHC-PELISA

Reactivity:Human, Mouse, Rat

Non-phospho-YAP1-S127 Rabbit pAb

Phospho-YAP1-S127 Rabbit mAb
AP1436

WBIF/ICCELISA

Reactivity:Human, Mouse, Rat

Phospho-YAP1-S127 Rabbit mAb

Secondary Antibodies (25)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb