제품 > 항체 > 단일클론항체(mAb)

[KO Validated] YAP1 Rabbit mAb (A19134)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and YAP1 knockout (KO) HeLa cells using [KO Validated] YAP1 Rabbit mAb (A19134) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] YAP1 Rabbit mAb (A19134)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] YAP1 Rabbit mAb (A19134) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] YAP1 Rabbit mAb (A19134) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KO Validated] YAP1 Rabbit mAb (A19134) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KO Validated] YAP1 Rabbit mAb (A19134) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] YAP1 Rabbit mAb (A19134) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] YAP1 Rabbit mAb (A19134) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] YAP1 Rabbit mAb (A19134)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] YAP1 Rabbit mAb (A19134) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U20S cells using [KO Validated] YAP1 Rabbit mAb (A19134) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using [KO Validated] YAP1 Rabbit mAb (A19134) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] YAP1 Rabbit mAb (A19134)

Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg YAP1 antibody (A19134). Western blot was performed from the immunoprecipitate using YAP1 antibody (A19134) at a dilution of 1:500.

Overview

Product name	[KO Validated] YAP1 Rabbit mAb
Catalog No.	A19134
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53477

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
Sequence	PTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL
Gene ID
Swiss Prot
Synonyms	YAP; YKI; COB1; YAP2; YAP-1; YAP65; P1
Calculated MW	36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
Observed MW	70kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:10000 - 1:40000 IHC-P 1:200 - 1:2000 IF/ICC 1:100 - 1:400 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Mus musculus, Homo sapiens) IHC(Homo sapiens) IHC(Rattus norvegicus, Mus musculus) WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] YAP1 Rabbit mAb images

Western blot - [KO Validated] YAP1 Rabbit mAb (A19134)

Immunohistochemistry - [KO Validated] YAP1 Rabbit mAb (A19134)

Immunofluorescence - [KO Validated] YAP1 Rabbit mAb (A19134)

Immunoprecipitation - [KO Validated] YAP1 Rabbit mAb (A19134)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

36238573

Title:

Pyrroloquinoline quinone modulates YAP-related anti-ferroptotic activity to protect against myocardial hypertrophy - PMC

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Journal of biomedical materials research. Part A

Application:

IF:

4.85

Species:

Mus musculus

PMID:

35603761

Title:

Synergistic effects of fluid shear stress and adhesion morphology on the apoptosis and osteogenesis of mesenchymal stem cells

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Frontiers in oncology

Application:

WB, IHC

IF:

5.738

Species:

Homo sapiens

PMID:

35359362

Title:

Exosomal CD44 Transmits Lymph Node Metastatic Capacity Between Gastric Cancer Cells via YAP-CPT1A-Mediated FAO Reprogramming

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

The Canadian journal of cardiology

Application:

IF:

6.61

Species:

Rattus norvegicus

PMID:

37541340

Title:

YAP facilitates NEDD4L-mediated ubiquitination and degradation of ACSL4 to alleviate ferroptosis in myocardial ischemia-reperfusion injury

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

The Canadian journal of cardiology

Application:

IHC

IF:

6.61

Species:

Rattus norvegicus

PMID:

37541340

Title:

YAP facilitates NEDD4L-mediated ubiquitination and degradation of ACSL4 to alleviate ferroptosis in myocardial ischemia-reperfusion injury

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Homo sapiens

PMID:

38816763

Title:

Low concentrations of saracatinib promote definitive endoderm differentiation through inhibition of FAK-YAP signaling axis - PMC

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Homo sapiens

PMID:

38816763

Title:

Low concentrations of saracatinib promote definitive endoderm differentiation through inhibition of FAK-YAP signaling axis - PMC

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Cell death and differentiation

Application:

IF:

12.4

Species:

Mus musculus

PMID:

38783090

Title:

MLKL promotes hepatocarcinogenesis through inhibition of AMPK-mediated autophagy

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IHC, WB

IF:

5.6

Species:

Mus musculus

PMID:

39040468

Title:

Knockout of C1q/tumor necrosis factor-related protein-9 aggravates cardiac fibrosis in diabetic mice by regulating YAP-mediated autophagy - PMC

References for [KO Validated] YAP1 Rabbit mAb (A19134)

Product:

[KO Validated] YAP1 Rabbit mAb

Journal:

Food & function

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38411344

Title:

A CDAHFD-induced mouse model mimicking human NASH in the metabolism of hepatic phosphatidylcholines and acyl carnitines

Publishing research using A19134? Please let us know so that we can cite the reference in this datasheet.

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제품 > 항체 > 단일클론항체(mAb)

[KO Validated] YAP1 Rabbit mAb (A19134)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

[KO Validated] YAP1 Rabbit mAb images

Product Protocols

항체 (11)

Secondary Antibodies (26)