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phospho-YAP1-S397 Rabbit pAb (AP0922)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using phospho-YAP1-S397 Rabbit pAb (AP0922) at 1:1000 dilution. NIH3T3 cells and C6 cells were treated by Sorbitol (0. 2M) at 37℃ for 60 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.

ABclonal:Immunofluorescence - phospho-YAP1-S397 Rabbit pAb (AP0922)

Immunofluorescence analysis of HeLa cells (treated with CA) and HeLa cells (untreated) using phospho-YAP1-S397 Rabbit pAb (AP0922) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	phospho-YAP1-S397 Rabbit pAb
Catalog No.	AP0922
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S397 of human YAP1 (NP_001123617.1).
Sequence	DESTD
Gene ID
Swiss Prot
Synonyms	YAP; YKI; COB1; YAP2; YAP-1; YAP65; phospho-YAP1-S397
Calculated MW	54kDa
Observed MW	75kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3 treated by Sorbitol, C6 treated by Sorbitol
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Rattus norvegicus)