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[KO Validated] LAMP2 Rabbit mAb (A25646)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from A549 cells using [KO Validated] LAMP2 Rabbit mAb (A25646) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:10s.

Western blot analysis of lysates from wild type (WT) and LAMP2 knockout (KO) HeLa cells using [KO Validated] LAMP2 Rabbit mAb (A25646) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] LAMP2 Rabbit mAb (A25646)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] LAMP2 Rabbit mAb (A25646) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] LAMP2 Rabbit mAb (A25646)

Confocal imaging of HeLa cells using [KO Validated] LAMP2 Rabbit mAb (A25646, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - [KO Validated] LAMP2 Rabbit mAb (A25646)

Flow cytometry: 1X10^6 knockout (KO) HeLa cells (negative control, left) and Hela cells (right) were intracellularly-stained with [KO Validated] LAMP2 Rabbit mAb (A25646, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 knockout (KO) HeLa cells (negative control, left) and Hela cells (right) were intracellularly-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or [KO Validated] LAMP2 Rabbit mAb (A25646, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-Rabbit pAb staining.

Overview

Product name	[KO Validated] LAMP2 Rabbit mAb
Catalog No.	A25646
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC54767

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 29-375 of human LAMP2 (NP_002285.1).
Sequence	LELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNF
Gene ID
Swiss Prot
Synonyms	DND; LAMPB; CD107b; LAMP-2; LGP-96; LGP110
Calculated MW	45kDa
Observed MW	100-130kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:20000 - 1:120000 IHC 1:200 - 1:2000 IF/ICC 1:200 - 1:800 FC (intra) 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	A549, HeLa
Cellular location	Cell membrane, Single-pass type I membrane protein, Endosome membrane, Lysosome membrane, Cytoplasmic vesicle, autophagosome membrane, extracellular exosome, extracellular space, late endosome, lysosomal lumen, lysosome, perinuclear region of cytoplasm, plasma membrane, trans-Golgi network.