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LAMP2 Rabbit pAb (A1961)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - LAMP2 Rabbit pAb (A1961)

Western blot analysis of various lysates, using LAMP2 antibody (A1961) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - LAMP2 Rabbit pAb (A1961)

Western blot analysis of Mouse kidney, using LAMP2 antibody (A1961) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - LAMP2 Rabbit pAb (A1961)

Immunohistochemistry analysis of paraffin-embedded human kidney using LAMP2 Rabbit pAb (A1961) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LAMP2 Rabbit pAb (A1961)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using LAMP2 Rabbit pAb (A1961) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - LAMP2 Rabbit pAb (A1961)

Immunofluorescence analysis of HeLa cells using LAMP2 Rabbit pAb (A1961) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameLAMP2 Rabbit pAb
Catalog No.A1961
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 29-375 of human LAMP2 (NP_002285.1).
SequenceLELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNF
Gene ID
Swiss Prot
SynonymsDND; LAMPB; CD107b; LAMP-2; LGP-96; LGP110
Calculated MW45kDa
Observed MW100-130kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSK-MEL-28, HeLa, Mouse kidney
Cellular locationCell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - LAMP2 Rabbit pAb (A1961)}

Western blot - LAMP2 Rabbit pAb (A1961)

Western blot analysis of various lysates, using LAMP2 antibody (A1961) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - LAMP2 Rabbit pAb (A1961)}

Western blot - LAMP2 Rabbit pAb (A1961)

Western blot analysis of Mouse kidney, using LAMP2 antibody (A1961) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - LAMP2 Rabbit pAb (A1961)}

Immunohistochemistry - LAMP2 Rabbit pAb (A1961)

Immunohistochemistry analysis of paraffin-embedded human kidney using LAMP2 Rabbit pAb (A1961) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LAMP2 Rabbit pAb (A1961)}

Immunohistochemistry - LAMP2 Rabbit pAb (A1961)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using LAMP2 Rabbit pAb (A1961) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - LAMP2 Rabbit pAb (A1961)}

Immunofluorescence - LAMP2 Rabbit pAb (A1961)

Immunofluorescence analysis of HeLa cells using LAMP2 Rabbit pAb (A1961) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1961? Please let us know so that we can cite the reference in this datasheet.

항체 (8)

ELISA 키트 (1)

Secondary Antibodies (26)