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LAMP2 Rabbit mAb (A0593)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LAMP2 Rabbit mAb (A0593)

Western blot analysis of lysates from A-549 cells, using LAMP2 Rabbit mAb (A0593) at 1：1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - LAMP2 Rabbit mAb (A0593)

Western blot analysis of various lysates using LAMP2 Rabbit mAb (A0593) at 1：1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - LAMP2 Rabbit mAb (A0593)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using LAMP2 Rabbit mAb (A0593) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LAMP2 Rabbit mAb (A0593)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using LAMP2 Rabbit mAb (A0593) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LAMP2 Rabbit mAb (A0593)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using LAMP2 Rabbit mAb (A0593) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameLAMP2 Rabbit mAb
Catalog No.A0593
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0274
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 311-410 of human LAMP2 (P13473).
SequenceFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQDCSADDDNFLVPIAVGAALAGVLILVLLAYFIGLKHHHAGYEQF
Gene ID
Swiss Prot
SynonymsDND; LAMPB; CD107b; LAMP-2; LGP-96; LGP110; LAMP2
Calculated MW45kDa
Observed MW100-120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA-549, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Cellular locationCell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - LAMP2 Rabbit mAb (A0593)}

Western blot - LAMP2 Rabbit mAb (A0593)

Western blot analysis of lysates from A-549 cells, using LAMP2 Rabbit mAb (A0593) at 1：1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - LAMP2 Rabbit mAb (A0593)}

Western blot - LAMP2 Rabbit mAb (A0593)

Western blot analysis of various lysates using LAMP2 Rabbit mAb (A0593) at 1：1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - LAMP2 Rabbit mAb (A0593)}

Immunohistochemistry - LAMP2 Rabbit mAb (A0593)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using LAMP2 Rabbit mAb (A0593) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LAMP2 Rabbit mAb (A0593)}

Immunohistochemistry - LAMP2 Rabbit mAb (A0593)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using LAMP2 Rabbit mAb (A0593) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LAMP2 Rabbit mAb (A0593)}

Immunohistochemistry - LAMP2 Rabbit mAb (A0593)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using LAMP2 Rabbit mAb (A0593) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0593? Please let us know so that we can cite the reference in this datasheet.

항체 (8)

[KO Validated] LAMP2 Rabbit pAb
A14017

ELISAWBIHC-P

Reactivity:Human, Mouse

[KO Validated] LAMP2 Rabbit pAb

LAMP2 Rabbit pAb
A1961

ELISAWBIHC-PIF/ICC

Reactivity:Human, Mouse

LAMP2 Rabbit pAb

ABflo® 647 Rabbit anti-Human LAMP2 mAb
A22217

IF/ICCFC(intra)

Reactivity:Human

ABflo® 647 Rabbit anti-Human LAMP2 mAb

ABflo® 488 Rabbit anti-Human LAMP2 mAb
A22216

IF/ICCFC(intra)

Reactivity:Human

ABflo® 488 Rabbit anti-Human LAMP2 mAb

ABflo® 594 Rabbit anti-Human LAMP2 mAb
A24189

FC(intra)

Reactivity:Human

ABflo® 594 Rabbit anti-Human LAMP2 mAb

[KO Validated] LAMP2 Rabbit mAb
A25646

WBIHC-PIF/ICCFC(intra)ELISA

Reactivity:Human

[KO Validated] LAMP2 Rabbit mAb

ABflo® 450 Rabbit anti-Human LAMP2 mAb
A26570

FC(intra)

Reactivity:Human

ABflo® 450 Rabbit anti-Human LAMP2 mAb

[KO Validated] LAMP2 Rabbit PolymAb®
A26781PM

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

[KO Validated] LAMP2 Rabbit PolymAb®

ELISA 키트 (1)

Human Human Lysosomal-associated membrane protein 2 (HLAMP-2) ELISA Kit
RK09182

Human Human Lysosomal-associated membrane protein 2 (HLAMP-2) ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)