제품 > 항체 > 단일클론항체(mAb)

MEK1/MEK2 Rabbit mAb (A4868)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using MEK1/MEK2 Rabbit mAb (A4868) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	MEK1/MEK2 Rabbit mAb
Catalog No.	A4868
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0292

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human MEK1/MEK2 (Q02750).
Sequence	MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAF
Gene ID
Swiss Prot
Synonyms	CFC3; MAPKK1; MEK1; MKK1; PRKMK1; MEK1/MEK2
Calculated MW	44kDa
Observed MW	42kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:5000 - 1:30000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, HepG2, Jurkat, Mouse brain
Cellular location	cytosol, early endosome, endoplasmic reticulum, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, nucleus, plasma membrane.
Customer validation	WB(Mus musculus, Homo sapiens, Gallus gallus, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

MEK1/MEK2 Rabbit mAb images

Western blot - MEK1/MEK2 Rabbit mAb (A4868)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Front Oncol

Application:

IF:

6.244

Species:

Mus musculus

PMID:

34790577

Title:

Anti-Tumor Activity of AZD4547 Against NTRK1 Fusion Positive Cancer Cells Through Inhibition of NTRKs

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Nucleic acids research

Application:

IF:

19.16

Species:

Homo sapiens

PMID:

36124682

Title:

Super-enhancer-controlled positive feedback loop BRD4/ERα–RET–ERα promotes ERα-positive breast cancer

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

36158676

Title:

Co-administration of MDR1 and BCRP or EGFR/PI3K inhibitors overcomes lenvatinib resistance in hepatocellular carcinoma - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Cancer biology & therapy

Application:

IF:

4.87

Species:

Homo sapiens

PMID:

36137220

Title:

PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Molecular cancer

Application:

IF:

41.44

Species:

Homo sapiens

PMID:

37408008

Title:

A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Gallus gallus

PMID:

38034812

Title:

Antagonistic effects of N-acetylcysteine on lead-induced apoptosis and oxidative stress in chicken embryo fibroblast cells - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

6.63

Species:

Rattus norvegicus

PMID:

37952827

Title:

Inhibition of Sema4D attenuates pressure overload-induced pathological myocardial hypertrophy via the MAPK/NF-B/NLRP3 pathways

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IF:

5.21

Species:

Homo sapiens

PMID:

36678513

Title:

Preclinical Studies of Chiauranib Show It Inhibits Transformed Follicular Lymphoma through the VEGFR2/ERK/STAT3 Signaling Pathway - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

38361605

Title:

CircZCCHC2 decreases pirarubicin sensitivity and promotes triple-negative breast cancer development via the miR-1200/TPR axis - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Mus musculus

PMID:

36709322

Title:

Polymeric immunoglobulin receptor deficiency exacerbates autoimmune hepatitis by inducing intestinal dysbiosis and barrier dysfunction - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Poultry science

Application:

IF:

4.01

Species:

Gallus gallus

PMID:

36621100

Title:

Ligusticum chuanxiong promotes the angiogenesis of preovulatory follicles (F1-F3) in late-phase laying hens

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

EBioMedicine

Application:

IF:

11.1

Species:

Homo sapiens

PMID:

38810561

Title:

Development of a PAK4-targeting PROTAC for renal carcinoma therapy: concurrent inhibition of cancer cell proliferation and enhancement of immune cell response - PMC

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Molecular immunology

Application:

IF:

3.6

Species:

Mus musculus

PMID:

38820902

Title:

Rosmarinic acid activates the Ras/Raf/MEK/ERK signaling pathway to regulate CD8+ T cells and autophagy to clear Chlamydia trachomatis in reproductive tract

References for MEK1/MEK2 Rabbit mAb (A4868)

Product:

MEK1/MEK2 Rabbit mAb

Journal:

Frontiers in microbiology

Application:

IF:

5.2

Species:

Homo sapiens

PMID:

39360313