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Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)

Western blot analysis of various lysates, using Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)at 1:3000 dilution (upper) or MEK1/MEK2 Rabbit mAb (A4868) at1:1000 dilution (lower). incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated by PMA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. C6 cells were treated by PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunoprecipitation - Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)

Immunoprecipitation of Phospho-MEK1/MEK2-S217/S221 from 1000 µg extracts of HeLa cells treated by PMA (200nM, 1h) was performed using 2 µg of Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349) at a dilution of 1:5000.

Overview

Product namePhospho-MEK1/MEK2-S217/S221 Rabbit mAb
Catalog No.AP1349
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55711
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
ImmunogenA synthetic phosphorylated peptide around S217 & S221 of human MEK1/MEK2 (NP_002746.1).
SequenceDSMAN
Gene ID
Swiss Prot
SynonymsMEK1/MEK2; Phospho-MEK1/MEK2-S217/S221
Calculated MW40kDa/43kDa/44kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IP 1μg-4μg antibody for 800μg-1000μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesHeLa treated by PMA, NIH/3T3 treated by PMA, C6 treated by PMA
Cellular locationnucleus, membrane, cytoskeleton, microtubule organizing center, spindle pole body, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)}

    Western blot - Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)

    Western blot analysis of various lysates, using Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)at 1:3000 dilution (upper) or MEK1/MEK2 Rabbit mAb (A4868) at1:1000 dilution (lower). incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated by PMA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. C6 cells were treated by PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 30 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Immunoprecipitation - Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)}

    Immunoprecipitation - Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349)

    Immunoprecipitation of Phospho-MEK1/MEK2-S217/S221 from 1000 µg extracts of HeLa cells treated by PMA (200nM, 1h) was performed using 2 µg of Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-MEK1/MEK2-S217/S221 Rabbit mAb (AP1349) at a dilution of 1:5000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)