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Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells using Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209) at 1:6000 dilution. HeLa cells were treated by PMA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product name	Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb
Catalog No.	AP0209
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S217 & S221 of human MAP2K1/MAP2K2MAP2K1 (NP_002746.1).
Sequence	SMANS
Gene ID
Swiss Prot
Synonyms	MEK1/MEK2; Phospho-MAP2K1-S217/MAP2K2-S221
Calculated MW	40kDa/43kDa/44kDa
Observed MW	44kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:7000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by PMA
Cellular location	cytosol, early endosome, endoplasmic reticulum, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, nucleus, plasma membrane.
Customer validation	WB(Rattus norvegicus, Mus musculus, Homo sapiens, Other) Co-IP(Homo sapiens)

Documents

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Research Area

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb images

Western blot - Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Medical science monitor

Application:

IF:

2.649

Species:

Rattus norvegicus

PMID:

30538214

Title:

Involvement of Activation of Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK) Signaling Pathway in Proliferation of Urethral Plate Fibroblasts in Finasteride-Induced Rat Hypospadias.

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Life Sciences

Application:

IF:

5.037

Species:

Mus musculus

PMID:

31811853

Title:

Glibenclamide ameliorates transplant-induced arteriosclerosis and inhibits macrophage migration and MCP-1 expression

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Journal Of Ethnopharmacology

Application:

IF:

4.36

Species:

Mus musculus

PMID:

33249246

Title:

Longan (Dimocarpus longan Lour.) Aril ameliorates cognitive impairment in AD mice induced by combination of D-gal/AlCl3 and an irregular diet via RAS/MEK/ERK signaling pathway

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Clin Res Hepatol Gastroenterol

Application:

IF:

2.947

Species:

Mus musculus

PMID:

34144219

Title:

Cellular Communication Network Factor 1 (CCN1) Knockdown Exerts a Protective Effect for Hepatic Ischemia/Reperfusion Injury by Deactivating the MEK/ERK pathway

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Nucleic acids research

Application:

IF:

19.16

Species:

Homo sapiens

PMID:

36124682

Title:

Super-enhancer-controlled positive feedback loop BRD4/ERα–RET–ERα promotes ERα-positive breast cancer

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

36158676

Title:

Co-administration of MDR1 and BCRP or EGFR/PI3K inhibitors overcomes lenvatinib resistance in hepatocellular carcinoma - PMC

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Homo sapiens

PMID:

35839564

Title:

EphA10 drives tumor progression and immune evasion by regulating the MAPK/ERK cascade in lung adenocarcinoma

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Cancer biology & therapy

Application:

IF:

4.87

Species:

Homo sapiens

PMID:

36137220

Title:

PSMD12 promotes the activation of the MEK-ERK pathway by upregulating KIF15 to promote the malignant progression of liver cancer - PMC

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

4.13

Species:

Homo sapiens

PMID:

37052291

Title:

Long non-coding RNA BLACAT2/miR-378a-3p/YY1 feedback loop promotes the proliferation, migration and invasion of uterine corpus endometrial carcinoma - PMC

References for Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (AP0209)

Product:

Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

6.63

Species:

Rattus norvegicus

PMID:

37952827