제품 > 항체 > 단일클론항체(mAb)

Mitofusin 2 Rabbit mAb (A19678)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Mitofusin 2 Rabbit mAb (A19678) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Mitofusin 2 Rabbit mAb (A19678)

Confocal imaging of paraffin-embedded Mouse brain tissue using Mitofusin 2 Rabbit mAb (A19678, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product name	Mitofusin 2 Rabbit mAb
Catalog No.	A19678
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0157

Background

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Mitofusin 2 (O95140).
Sequence	MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVA
Gene ID
Swiss Prot
Synonyms	HSG; MARF; CMT2A; CPRP1; CMT2A2; HMSN6A; CMT2A2A; CMT2A2B; Mitofusin 2
Calculated MW	86kDa
Observed MW	80kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:100 - 1:400 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Raji, MCF7, 293T, NIH/3T3, Mouse brain, Rat heart, Rat brain
Cellular location	Mitochondrion outer membrane, Multi-pass membrane protein.
Customer validation	WB(Rattus norvegicus, Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus, Gallus gallus) IHC(Mus musculus, Rattus norvegicus) IF(Mus musculus, Rattus norvegicus) IF(Gallus gallus)

Documents

Certificate of Compliance

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Lot number

Research Area

Mitofusin 2 Rabbit mAb images

Western blot - Mitofusin 2 Rabbit mAb (A19678)

Immunofluorescence - Mitofusin 2 Rabbit mAb (A19678)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Pharmacology

Application:

IF:

2.9

Species:

Rattus norvegicus

PMID:

33691319

Title:

Schisandrin Restores the Amyloid β-Induced Impairments on Mitochondrial Function, Energy Metabolism, Biogenesis, and Dynamics in Rat Primary Hippocampal Neurons

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

J Mol Cell Biol

Application:

IF:

5.3

Species:

Mus musculus

PMID:

33988719

Title:

Knockdown of insulin-like growth factor 2 gene disrupts mitochondrial functions in the liver

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Arch Biochem Biophys

Application:

IF:

3.8

Species:

Mus musculus

PMID:

34153296

Title:

Glucose fluctuation accelerates cardiac injury of diabetic mice via sodium-dependent glucose cotransporter 1 (SGLT1)

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Food Funct

Application:

IF:

5.1

Species:

Mus musculus

PMID:

34821902

Title:

Ganoderic acid improves 5-fluorouracil-induced cognitive dysfunction in mice

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Nat Commun

Application:

IF:

16.6

Species:

Rattus norvegicus

PMID:

34815412

Title:

Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Infection and immunity

Application:

IF:

2.9

Species:

Homo sapiens

PMID:

34370506

Title:

Mitochondrial fusion mediated by mitofusin 1 regulates macrophage mycobactericidal activity by enhancing autophagy

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Biochemistry and cell biology = Biochimie et biologie cellulaire

Application:

IF:

2.4

Species:

Rattus norvegicus

PMID:

33259229

Title:

Knockdown of SGLT1 prevents the apoptosis of cardiomyocytes induced by glucose fluctuation via relieving oxidative stress and mitochondrial dysfunction

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

35524750

Title:

Mitophagy induced by UMI-77 preserves mitochondrial fitness in renal tubular epithelial cells and alleviates renal fibrosis

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

6.2

Species:

Mus musculus

PMID:

36399995

Title:

Puerarin prevents cadmium-induced mitochondrial fission in AML-12 cells via Sirt1-dependent pathway

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36583724

Title:

Mice harboring a R133L heterozygous mutation in LMNA exhibited ectopic lipid accumulation, aging, and mitochondrial dysfunction in adipose tissue

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Respiratory research

Application:

IF:

5.8

Species:

Mus musculus

PMID:

36922854

Title:

Inhibition of fatty acid synthase protects obese mice from acute lung injury via ameliorating lung endothelial dysfunction - PMC

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Acta pharmacologica Sinica

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

36859596

Title:

Astragalin ameliorates renal injury in diabetic mice by modulating mitochondrial quality control via AMPK-dependent PGC1α pathway

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Journal of ginseng research

Application:

IF:

6.8

Species:

Mus musculus

PMID:

36644390

Title:

Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive …

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

International urology and nephrology

Application:

IF:

1.8

Species:

Homo sapiens

PMID:

36749472

Title:

GDF11, a target of miR-32-5p, suppresses high-glucose-induced mitochondrial dysfunction and apoptosis in HK-2 cells through PI3K/AKT signaling activation

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

3.8

Species:

Sus scrofa

PMID:

37031286

Title:

The apoptotic effects of soybean agglutinin were induced through three different signal pathways by down-regulating cytoskeleton proteins in IPEC-J2 cells - PMC

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

EMBO Reports

Application:

IF:

7.7

Species:

Rattus norvegicus

PMID:

37358015

Title:

Enhancing Spns2/S1P in macrophages alleviates hyperinflammation and prevents immunosuppression in sepsis

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

WB, IHC, IF

IF:

7.7

Species:

Mus musculus, Rattus norvegicus

PMID:

37237889

Title:

Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress - PMC

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Journal of molecular and cellular cardiology

Application:

IF:

4.9

Species:

Mus musculus

PMID:

36509022

Title:

Klf4 deficiency exacerbates myocardial ischemia/reperfusion injury in mice via enhancing ROCK1/DRP1 pathway-dependent mitochondrial fission

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Environmental toxicology

Application:

IF:

4.5

Species:

Homo sapiens,Rattus norvegicus

PMID:

38511876

Title:

Arsenic exposure induced renal fibrosis via regulation of mitochondrial dynamics and the NLRP3TGF1/SMAD signaling pathway

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Homo sapiens

PMID:

38539195

Title:

Human periodontal ligament stem cell sheets activated by graphene oxide quantum dots repair periodontal bone defects by promoting mitochondrial

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Journal of translational medicine

Application:

IF:

8.4

Species:

Homo sapiens,Mus musculus

PMID:

37798747

Title:

TRPA1 promotes cisplatin-induced acute kidney injury via regulating the endoplasmic reticulum stress-mitochondrial damage - PMC

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

The Journal of clinical investigation

Application:

IF:

13.3

Species:

Mus musculus

PMID:

38702076

Title:

Intermittent glucocorticoid treatment improves muscle metabolism via the PGC1/Lipin1 axis in an aging-related sarcopenia model

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

4.2

Species:

Homo sapiens

PMID:

38866362

Title:

Diosgenin attenuates nonalcoholic hepatic steatosis through the hepatic SIRT1/PGC-1 pathway

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Frontiers of medicine

Application:

IF:

3.9

Species:

Mus musculus

PMID:

38743133

Title:

lncRNA ZNF593-AS inhibits cardiac hypertrophy and myocardial remodeling by upregulating Mfn2 expression

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

38583309

Title:

New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

Application:

IF:

3.9

Species:

Gallus gallus

PMID:

39089429

Title:

Hesperidin Alleviatesterbuthylazine-Induced Ferroptosisvia Maintenance of Mitochondria-Associated Endoplasmic Reticulum Membrane Integrity in Chicken

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

International journal of cardiology

Application:

IF:

3.2

Species:

Mus musculus

PMID:

38723908

Title:

USP7 cardiomyocyte specific knockout causes disordered mitochondrial biogenesis and dynamics and early neonatal lethality in mice

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Pharmacological research

Application:

IF:

9.1

Species:

Homo sapiens,Mus musculus

PMID:

38815879

Title:

USP7/PGC1/PPAR Axis Promotes Cardiometabolic Disorders and Mitochondrial Homeostasis Dysfunction in Diabetic Mice

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

7.6

Species:

Mus musculus

PMID:

38423272

Title:

Inhalation exposure to polystyrene nanoplastics induces chronic obstructive pulmonary disease-like lung injury in mice through multi-dimensional assessment

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

Cell & bioscience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

39543767

Title:

Ancestral retrovirus envelope protein ERVWE1 upregulates circ_0001810, a potential biomarker for schizophrenia, and induces neuronal mitochondrial dysfunction via activating AK2 - PMC

References for Mitofusin 2 Rabbit mAb (A19678)

Product:

Mitofusin 2 Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

39837016