제품 > 항체 > 다중클론항체(pAb)

N-Cadherin Rabbit pAb (A0432)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using N-Cadherin Rabbit pAb (A0432) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	N-Cadherin Rabbit pAb
Catalog No.	A0432
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 500-800 of human N-Cadherin (NP_001783.2).
Sequence	NPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNILKYDEEGGGEEDQDYDLSQLQQPDTVEPDA
Gene ID
Swiss Prot
Synonyms	CDHN; NCAD; ACOGS; ADHD8; CD325; ARVD14; CDw325; N-Cadherin
Calculated MW	100kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, Mouse brain, Mouse heart, Rat heart
Cellular location	Cell membrane, Single-pass type I membrane protein.
Customer validation	IF(Homo sapiens) WB(Homo sapiens, Mus musculus, Rattus norvegicus) ELISA(Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

N-Cadherin Rabbit pAb images

Western blot - N-Cadherin Rabbit pAb (A0432)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for N-Cadherin Rabbit pAb (A0432)

Product:

N-Cadherin Rabbit pAb

Journal:

Clinical and translational medicine

Application:

IF:

10.6

Species:

Homo sapiens

PMID:

36394206

Title:

Alternative splicing of HSPA12A pre-RNA by SRSF11 contributes to metastasis potential of colorectal cancer - PMC

References for N-Cadherin Rabbit pAb (A0432)

Product:

N-Cadherin Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

WB, ELISA

IF:

15.1

Species:

Homo sapiens, Mus musculus, Rattus norvegicus

PMID:

37566765

Title:

Nuclear Actin Polymerization Regulates Cell EpithelialMesenchymal Transition

References for N-Cadherin Rabbit pAb (A0432)

Product:

N-Cadherin Rabbit pAb

Journal:

Environmental toxicology

Application:

IHC

IF:

4.5

Species:

Mus musculus

PMID:

37665156

Title:

FOXA2 suppresses gallbladder carcinoma cell migration, invasion, and epithelialmesenchymal transition by targeting SERPINB5

References for N-Cadherin Rabbit pAb (A0432)

Product:

N-Cadherin Rabbit pAb

Journal:

Environmental toxicology

Application:

IF:

4.5

Species:

Homo sapiens

PMID:

37665156

Title:

FOXA2 suppresses gallbladder carcinoma cell migration, invasion, and epithelialmesenchymal transition by targeting SERPINB5

References for N-Cadherin Rabbit pAb (A0432)

Product:

N-Cadherin Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.1

Species:

Homo sapiens

PMID:

37002201

Title:

Nodal promotes colorectal cancer survival and metastasis through regulating SCD1-mediated ferroptosis resistance - PMC

References for N-Cadherin Rabbit pAb (A0432)

Product:

N-Cadherin Rabbit pAb

Journal:

Journal of translational medicine

Application:

IF:

8.4

Species:

Homo sapiens

PMID:

39402642

Title:

m6A-modified circXPO1 accelerates colorectal cancer progression via interaction with FMRP to promote WWC2 mRNA decay - PMC

References for N-Cadherin Rabbit pAb (A0432)

Product:

N-Cadherin Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

7.7

Species:

Homo sapiens

PMID:

37956801

Title:

Engineering alginate/carboxymethylcellulose scaffolds to establish liver cancer spheroids: Evaluation of molecular variances between 2D and 3D models

Publishing research using A0432? Please let us know so that we can cite the reference in this datasheet.

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제품 > 항체 > 다중클론항체(pAb)

N-Cadherin Rabbit pAb (A0432)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

N-Cadherin Rabbit pAb images

Product Protocols

항체 (3)

재조합 단백질 (1)

Secondary Antibodies (25)