제품 > 항체 > 다중클론항체(pAb)

NQO1 Rabbit pAb (A0047)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using NQO1 Rabbit pAb (A0047) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from Mouse liver, using NQO1 Rabbit pAb (A0047) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates, using NQO1 Rabbit pAb (A0047) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A0047)

Immunofluorescence analysis of NIH/3T3 cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	NQO1 Rabbit pAb
Catalog No.	A0047
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human NQO1 (NP_000894.1).
Sequence	MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
Gene ID
Swiss Prot
Synonyms	DTD; QR1; DHQU; DIA4; NMOR1; NMORI; NQO1
Calculated MW	31kDa
Observed MW	31kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HepG2, MCF7, HeLa, Mouse liver
Cellular location	Cytoplasm.
Customer validation	(Huh7.5.1,HepG2) WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Sus scrofa) immunoblotting(Homo sapiens) IF(Sus scrofa)

Documents

Certificate of Compliance

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Lot number

Research Area

NQO1 Rabbit pAb images

Western blot - NQO1 Rabbit pAb (A0047)

Immunofluorescence - NQO1 Rabbit pAb (A0047)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0047

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Reviews List

Product Protocols

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Cancer letters

Application:

IF:

9.1

Species:

Mus musculus

PMID:

30401637

Title:

Diet-induced hepatic steatosis activates Ras to promote hepatocarcinogenesis via CPT1α.

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Oxidative Medicine and Cellular Longevity

Application:

IF:

Species:

Homo sapiens

PMID:

31687081

Title:

Bovine Herpesvirus 1 Productive Infection Led to Inactivation of Nrf2 Signaling through Diverse Approaches

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Oxidative Medicine and Cellular Longevity

Application:

IF:

Species:

Homo sapiens

PMID:

31687081

Title:

Bovine Herpesvirus 1 Productive Infection Led to Inactivation of Nrf2 Signaling through Diverse Approaches

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Life Sciences

Application:

IF:

5.2

Species:

Mus musculus

PMID:

31863770

Title:

Role of diets and exercise in ameliorating obesity-related hepatic steatosis: Insights at the microRNA-dependent thyroid hormone synthesis and action

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Redox Biology

Application:

IF:

10.7

Species:

Mus musculus

PMID:

31926620

Title:

Triggering apoptosis by oroxylin A through caspase-8 activation and p62/SQSTM1 proteolysis

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Toxicology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

32014472

Title:

NLRP3 inflammasome inhibition attenuates subacute neurotoxicity induced by acrylamide in vitro and in vivo

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Aging-us

Application:

IF:

3.9

Species:

Mus musculus

PMID:

32365333

Title:

Lycopene prevents carcinogen-induced cutaneous tumor by enhancing activation of the Nrf2 pathway through p62-triggered autophagic Keap1 degradation.

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Acta Histochem

Application:

IF:

2.3

Species:

Homo sapiens

PMID:

33068964

Title:

Silibinin ameliorats H2O2-induced cell apoptosis and oxidative stress response by activating Nrf2 signaling in trophoblast cells

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Cell Stress Chaperones

Application:

IF:

3.3

Species:

Rattus norvegicus

PMID:

33629253

Title:

NIX protein enhances antioxidant capacity of and reduces the apoptosis induced by HSP90 inhibitor luminespib/NVP-AUY922 in PC12 cells

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Drug Des Devel Ther

Application:

IF:

4.7

Species:

Mus musculus

PMID:

34321869

Title:

Baicalin Alleviates Oxidative Stress and Inflammation in Diabetic Nephropathy via Nrf2 and MAPK Signaling Pathway

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

34454174

Title:

Tanshinone IIA protects human coronary artery endothelial cells from ferroptosis by activating the NRF2 pathway

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.7

Species:

Homo sapiens

PMID:

36139869

Title:

DHPA Protects SH-SY5Y Cells from Oxidative Stress-Induced Apoptosis via Mitochondria Apoptosis and the Keap1/Nrf2/HO-1 Signaling Pathway - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

35849971

Title:

Sarmentosin promotes USP17 and regulates Nrf2-mediated mitophagy and cellular oxidative stress to alleviate APAP-induced acute liver failure

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Mus musculus

PMID:

35658231

Title:

Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

36584418

Title:

Modified rougan decoction alleviates lipopolysaccharide-enrofloxacin-induced hepatotoxicity via activating the Nrf2/ARE pathway in chicken - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Food & function

Application:

IF:

5.1

Species:

Mus musculus

PMID:

36786681

Title:

Confusoside, a dihydrochalcone glucoside, prevents acetaminophen-induced liver injury by modulating the Keap1-Nrf2/Nf-κB/Caspase signaling pathway

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

37245303

Title:

Glycyrrhizin protects against particulate matter-induced lung injury via regulation of endoplasmic reticulum stress and NLRP3 inflammasome-mediated pyroptosis …

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Mus musculus

PMID:

37063298

Title:

Rev-erbα agonist SR9009 protects against cerebral ischemic injury through mechanisms involving Nrf2 pathway - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Pathogens (Basel, Switzerland)

Application:

IF:

3.7

Species:

Mus musculus

PMID:

37375489

Title:

Oridonin Inhibits Mycobacterium marinum Infection-Induced Oxidative Stress In Vitro and In Vivo - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Mus musculus

PMID:

37646040

Title:

miR-200a attenuated oxidative stress, inflammation, and apoptosis in dextran sulfate sodium-induced colitis through activation of Nrf2 - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Cell reports. Medicine

Application:

immunoblotting

IF:

11.7

Species:

Homo sapiens

PMID:

38052215

Title:

Tumor-immune microenvironment and NRF2 associate with clinical efficacy of PD-1 blockade combined with chemotherapy in lung squamous cell carcinoma

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IF:

6.3

Species:

Sus scrofa

PMID:

38584279

Title:

Dietary fat supplementation relieves cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis in pigs - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

WB, IF

IF:

7.7

Species:

Sus scrofa

PMID:

36565836

Title:

Sodium butyrate protects against rotavirus-induced intestinal epithelial barrier damage by activating AMPK-Nrf2 signaling pathway in IPEC-J2 cells

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36461597

Title:

Fibroblast growth factor 10 protects against particulate matter-induced lung injury by inhibiting oxidative stress-mediated pyroptosis via the PI3K/Akt/Nrf2 signaling

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Inflammopharmacology

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38526770

Title:

Myricanol attenuates sepsis-induced inflammatory responses by nuclear factor erythroid 2-related factor 2 signaling and nuclear factor kappa B/mitogen-activated

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38692017

Title:

FGF10 protects against particulate matter-induced lung injury by inhibiting ferroptosis via Nrf2-dependent signaling

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Chemico-biological interactions

Application:

IF:

4.7

Species:

Mus musculus

PMID:

38705442

Title:

Paeoniflorin attenuates particulate matter-induced acute lung injury by inhibiting oxidative stress and NLRP3 inflammasome-mediated pyroptosis through activation of

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Rattus norvegicus

PMID:

38737244

Title:

Dihydroquercetin regulates HIF-1/AKT/NR2B signalling to improve impaired brain function in rats with metabolic syndrome - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

38782310

Title:

Untargeted serum and gastric metabolomics and network pharmacology analysis reveal the superior efficacy of zingiberis rhizoma recens-/euodiae fructus-processed

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Microorganisms

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38930573

Title:

Sulforaphane Inhibits Oxidative Stress and May Exert Anti-Pyroptotic Effects by Modulating NRF2/NLRP3 Signaling Pathway in Mycobacterium tuberculosis-Infected Macrophages - PMC

References for NQO1 Rabbit pAb (A0047)

Product:

NQO1 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.7

Species:

Mus musculus

PMID:

39079388