PE Mouse anti-Human CD3 mAb (A22795)

Reviews (2)Publication (1) Datasheet

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE Mouse anti-Human CD3 mAb (A22795)

Flow cytometry:1X10^6 Human PBMC were surface-stained with PE Mouse anti-Human CD3 mAb (A22795, orange line) or PE Mouse IgG1 isotype control mAb (A22878, blue line).

Review

Overview

Product name	PE Mouse anti-Human CD3 mAb
Catalog No.	A22795
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1
CloneNo.	AMC0533

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

Immunogen information

Immunogen	Recombinant protein of human CD3.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	T3E; TCRE; IMD18; CD3epsilon
Calculated MW	23kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 10 μL/Test
Storage buffer	Store at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.1% Proclin300, 0.2% BSA, pH7.2.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein.
Customer validation	(Human PBMC, Human PBMCs) FC(Homo sapiens)

Research Area

PE Mouse anti-Human CD3 mAb images

Flow CytoMetry - PE Mouse anti-Human CD3 mAb (A22795)

Flow cytometry:1X10^6 Human PBMC were surface-stained with PE Mouse anti-Human CD3 mAb (A22795, orange line) or PE Mouse IgG1 isotype control mAb (A22878, blue line).

* For research use only. Not for therapeutic or diagnostic purposes.