제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human PU.1/SPI1 mAb (A26564)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE Rabbit anti-Human PU.1/SPI1 mAb (A26564)

Flow cytometry: 1X10^6 HAP1 cells (negative control, left) and THP-1 cells (right) were intracellularly-stained with PE Rabbit anti-Human PU.1/SPI1 mAb (A26564, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product namePE Rabbit anti-Human PU.1/SPI1 mAb
Catalog No.A26564
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63111-PE
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PU.1/SPI1 (NP_003111.2)
SequenceMLQACKMEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPM
Gene ID
Swiss Prot
SynonymsOF; PU.1; AGM10; SFPI1; SPI-1; SPI-A
Calculated MW31kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationNucleus.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Flow CytoMetry - PE Rabbit anti-Human PU.1/SPI1 mAb (A26564)}

Flow CytoMetry - PE Rabbit anti-Human PU.1/SPI1 mAb (A26564)

Flow cytometry: 1X10^6 HAP1 cells (negative control, left) and THP-1 cells (right) were intracellularly-stained with PE Rabbit anti-Human PU.1/SPI1 mAb (A26564, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (5)

Secondary Antibodies (26)