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PKM1/2 Rabbit pAb (A13905)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PKM1/2 Rabbit pAb (A13905)

Western blot analysis of various lysates using PKM1/2 Rabbit pAb (A13905) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)

Immunohistochemistry analysis of paraffin-embedded Rat lung using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)

Immunohistochemistry analysis of paraffin-embedded Human colon using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - PKM1/2 Rabbit pAb (A13905)

Immunofluorescence analysis of L929 cells using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PKM1/2 Rabbit pAb (A13905)

Immunofluorescence analysis of U2OS cells using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePKM1/2 Rabbit pAb
Catalog No.A13905
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 11-221 of PKM1/2(NP_001193728.1).
SequenceAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAV
Gene ID
Swiss Prot
SynonymsPK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; PKM1/2
Calculated MW58kDa
Observed MW60kDa/58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, Mouse brain, Mouse heart, Mouse thymus, Rat brain, HeLa, Hep G2, 293T
Cellular locationCytoplasm, Nucleus.

Documents

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ABclonal:Western blot - PKM1/2 Rabbit pAb (A13905)}

Western blot - PKM1/2 Rabbit pAb (A13905)

Western blot analysis of various lysates using PKM1/2 Rabbit pAb (A13905) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)}

Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)}

Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)

Immunohistochemistry analysis of paraffin-embedded Rat lung using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)}

Immunohistochemistry - PKM1/2 Rabbit pAb (A13905)

Immunohistochemistry analysis of paraffin-embedded Human colon using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - PKM1/2 Rabbit pAb (A13905)}

Immunofluorescence - PKM1/2 Rabbit pAb (A13905)

Immunofluorescence analysis of L929 cells using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PKM1/2 Rabbit pAb (A13905)}

Immunofluorescence - PKM1/2 Rabbit pAb (A13905)

Immunofluorescence analysis of U2OS cells using PKM1/2 Rabbit pAb (A13905) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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