제품 > 항체 > 단일클론항체(mAb)

PKM2-specific Rabbit mAb (A20991)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using PKM2-specific Rabbit mAb (A20991) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - PKM2-specific Rabbit mAb (A20991)

Immunofluorescence analysis of A-204 cells using PKM2-specific Rabbit mAb (A20991) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using PKM2-specific Rabbit mAb (A20991) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - PKM2-specific Rabbit mAb (A20991)

Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg PKM2-specific Rabbit mAb (A20991). Western blot was performed from the immunoprecipitate using PKM2-specific Rabbit mAb (A20991) at a dilution of 1:1000.

Overview

Product name	PKM2-specific Rabbit mAb
Catalog No.	A20991
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC50919

Background

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 380-480 of human PKM2-specific (NP_002645.3).
Sequence	LIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQE
Gene ID
Swiss Prot
Synonyms	PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; PKM2-specific
Calculated MW	58kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	HeLa, NIH/3T3, C6
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Other)

Documents

Certificate of Compliance

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Lot number

Research Area

PKM2-specific Rabbit mAb images

Western blot - PKM2-specific Rabbit mAb (A20991)

Immunofluorescence - PKM2-specific Rabbit mAb (A20991)

Immunoprecipitation - PKM2-specific Rabbit mAb (A20991)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for PKM2-specific Rabbit mAb (A20991)

Product:

PKM2-specific Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.7

Species:

Homo sapiens

PMID:

38134863

Title:

Targeted metabolomics reveals PFKFB3 as a key target for elemene-mediated inhibition of glycolysis in prostate cancer cells

References for PKM2-specific Rabbit mAb (A20991)

Product:

PKM2-specific Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

Species:

Homo sapiens

PMID:

38200452

Title:

PRMT3 methylates HIF-1 to enhance the vascular calcification induced by chronic kidney disease - PMC

References for PKM2-specific Rabbit mAb (A20991)

Product:

PKM2-specific Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

Species:

Homo sapiens

PMID:

38671369

Title:

Ginsenoside Rh2 shifts tumor metabolism from aerobic glycolysis to oxidative phosphorylation through regulating the HIF1-/PDK4 axis in non-small cell lung cancer - PMC

References for PKM2-specific Rabbit mAb (A20991)

Product:

PKM2-specific Rabbit mAb

Journal:

Clinical and translational medicine

Application:

IF:

10.6

Species:

Mus musculus

PMID:

38616702