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PKM1-specific Rabbit pAb (A18800)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PKM1-specific Rabbit pAb (A18800)

Western blot analysis of various lysates using PKM1-specific Rabbit pAb (A18800) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - PKM1-specific Rabbit pAb (A18800)

Western blot analysis of various lysates using PKM1-specific Rabbit pAb (A18800) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)

Immunohistochemistry analysis of paraffin-embedded rat heart using PKM1-specific Rabbit pAb (A18800) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)

Immunohistochemistry analysis of paraffin-embedded human liver using PKM1-specific Rabbit pAb (A18800) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PKM1-specific Rabbit pAb (A18800) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PKM1-specific Rabbit pAb (A18800)

Immunofluorescence analysis of HeLa cells using PKM1-specific Rabbit pAb (A18800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PKM1-specific Rabbit pAb (A18800)

Immunofluorescence analysis of NIH/3T3 cells using PKM1-specific Rabbit pAb (A18800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePKM1-specific Rabbit pAb
Catalog No.A18800
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 350-450 of human PKM1-specific (NP_001193728.1).
SequenceGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAMFHRKLFEELVRASSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSAHQVARYR
Gene ID
Swiss Prot
SynonymsPK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; PKM1-specific
Calculated MW58kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse skeletal muscle, Mouse heart, Rat skeletal muscle
Cellular locationCytoplasm, Nucleus

Documents

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ABclonal:Western blot - PKM1-specific Rabbit pAb (A18800)}

Western blot - PKM1-specific Rabbit pAb (A18800)

Western blot analysis of various lysates using PKM1-specific Rabbit pAb (A18800) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - PKM1-specific Rabbit pAb (A18800)}

Western blot - PKM1-specific Rabbit pAb (A18800)

Western blot analysis of various lysates using PKM1-specific Rabbit pAb (A18800) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)}

Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)

Immunohistochemistry analysis of paraffin-embedded rat heart using PKM1-specific Rabbit pAb (A18800) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)}

Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)

Immunohistochemistry analysis of paraffin-embedded human liver using PKM1-specific Rabbit pAb (A18800) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)}

Immunohistochemistry - PKM1-specific Rabbit pAb (A18800)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PKM1-specific Rabbit pAb (A18800) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PKM1-specific Rabbit pAb (A18800)}

Immunofluorescence - PKM1-specific Rabbit pAb (A18800)

Immunofluorescence analysis of HeLa cells using PKM1-specific Rabbit pAb (A18800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PKM1-specific Rabbit pAb (A18800)}

Immunofluorescence - PKM1-specific Rabbit pAb (A18800)

Immunofluorescence analysis of NIH/3T3 cells using PKM1-specific Rabbit pAb (A18800) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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