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Phospho-PKM2-Y105 Rabbit pAb (AP0924)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PKM2-Y105 Rabbit pAb (AP0924)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKM2-Y105 Rabbit pAb (A13905). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Phospho-PKM2-Y105 Rabbit pAb (AP0924)

Immunofluorescence analysis of HUVEC cells using Phospho-PKM2-Y105 Rabbit pAb (AP0924) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-PKM2-Y105 Rabbit pAb
Catalog No.AP0924
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
ImmunogenA synthetic phosphorylated peptide around Y105 of human PKM (NP_002645.3).
SequenceILYRP
Gene ID
Swiss Prot
SynonymsPK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; Phospho-PKM2-Y105
Calculated MW58kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesNIH/3T3
Cellular locationCytoplasm, Nucleus.

Documents

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ABclonal:Western blot - Phospho-PKM2-Y105 Rabbit pAb (AP0924)}

Western blot - Phospho-PKM2-Y105 Rabbit pAb (AP0924)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKM2-Y105 Rabbit pAb (A13905). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Phospho-PKM2-Y105 Rabbit pAb (AP0924)}

Immunofluorescence - Phospho-PKM2-Y105 Rabbit pAb (AP0924)

Immunofluorescence analysis of HUVEC cells using Phospho-PKM2-Y105 Rabbit pAb (AP0924) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)