Reviews (2)Publications (19) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-Y15 Rabbit pAb (A0220). HeLa cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of various lysates using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded human breast cancer using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg Phospho-CDK1-Y15 pAb (AP0016). Western blot was performed from the immunoprecipitate using Phospho-CDK1-Y15 pAb (AP0016) at a dilution of 1:1000. HT-29 cells were treated by Serum-starvation overnight at 37℃.
| Product name | Phospho-CDK1-Y15 Rabbit pAb |
|---|---|
| Catalog No. | AP0016 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around Y15 of human CDK1 (NP_001777.1). |
|---|---|
| Sequence | GTYGV |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CDC2; CDC28A; P34CDC2; Phospho-CDK1-Y15 |
| Calculated MW | 34kDa |
| Observed MW | 34kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | HeLa treated by nocodazole, NIH/3T3 treated by nocodazole, C6 treated by nocodazole |
| Cellular location | Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle. |
| Customer validation | (H460,A549, HeLa) WB(Homo sapiens, Mus musculus, Eriocheir sinensis, Mus musculus) IF(Homo sapiens, Mus musculus) IP(Homo sapiens) |
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