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Phospho-DRP1-S616 Rabbit pAb (AP0849)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using Phospho-DRP1-S616 Rabbit pAb (AP0849) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-DRP1-S616 Rabbit pAb
Catalog No.	AP0849
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S616 of human DRP1 (NP_036192.2).
Sequence	PASPQ
Gene ID
Swiss Prot
Synonyms	DLP1; DRP1; DVLP; EMPF; OPA5; EMPF1; DYMPLE; HDYNIV; Phospho-DRP1-S616
Calculated MW	82kDa
Observed MW	83kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Calyculin A
Cellular location	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus, Membrane, Mitochondrion outer membrane, Peripheral membrane protein, Peroxisome, clathrin-coated pit, cytosol, secretory vesicle, synaptic vesicle membrane.
Customer validation	WB(Homo sapiens, Rattus norvegicus, Mus musculus, Mus musculus) IF(Rattus norvegicus) IHC(Mus musculus, Homo sapiens) mIHC(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-DRP1-S616 Rabbit pAb images

Western blot - Phospho-DRP1-S616 Rabbit pAb (AP0849)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Int J Oral Sci

Application:

IF:

6.344

Species:

Homo sapiens

PMID:

34475379

Title:

Porphyromonas gingivalis infection promotes mitochondrial dysfunction through Drp1-dependent mitochondrial fission in endothelial cells

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Nat Commun

Application:

IF:

14.919

Species:

Rattus norvegicus

PMID:

34815412

Title:

Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Aging

Application:

IF:

5.95

Species:

Homo sapiens

PMID:

36626243

Title:

Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female granulosa cells and mouse oocytes - PMC

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Mus musculus

PMID:

37196543

Title:

Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Neuroscience letters

Application:

IF:

3.19

Species:

Rattus norvegicus

PMID:

37926293

Title:

Transfer of massive mitochondria from astrocytes reduce propofol neurotoxicity

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Mus musculus

PMID:

38107281

Title:

Docosahexaenoic acid alleviates LPS-induced cytotoxicity in HL-1 cardiac cells via improving stress-induced mitochondrial fragmentation - PMC

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

17.52

Species:

Homo sapiens

PMID:

36031388

Title:

MetabolismBased Molecular Subtyping Endows Effective Ketogenic Therapy in p53Mutant Colon Cancer

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Pharmacological research

Application:

IF:

10.33

Species:

Homo sapiens, Mus musculus

PMID:

36813093

Title:

m6A methylation-induced NR1D1 ablation disrupts the HSC circadian clock and promotes hepatic fibrosis

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

PloS one

Application:

WB, IF

IF:

3.75

Species:

Rattus norvegicus

PMID:

36315519

Title:

2-Bromopalmitate decreases spinal inflammation and attenuates oxaliplatin-induced neuropathic pain via reducing Drp1-mediated mitochondrial dysfunction - PMC

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

WB, IF

IF:

5.29

Species:

Rattus norvegicus

PMID:

35689386

Title:

Glycogen synthase kinase-3 inhibition decreases inflammation and relieves cancer induced bone pain via reducing Drp1-mediated mitochondrial damage - PMC

References for Phospho-DRP1-S616 Rabbit pAb (AP0849)

Product:

Phospho-DRP1-S616 Rabbit pAb

Journal:

Journal of molecular and cellular cardiology

Application:

IF:

5.76

Species:

Mus musculus

PMID:

36509022