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Phospho-MEK1-S298 Rabbit mAb (AP1020)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Phospho-MEK1-S298 Rabbit mAb (AP1020)

Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (AP1020) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Overview

Product namePhospho-MEK1-S298 Rabbit mAb
Catalog No.AP1020
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1565
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
ImmunogenA synthetic phosphorylated peptide around S298 of human Phospho-MEK1-S298 (Q02750).
SequencePLSSY
Gene ID
Swiss Prot
SynonymsMEL; Phospho-MEK1-S298
Calculated MW43kDa
Observed MW45kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, C6, HeLa treated by PMA, C6 treated by PMA
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.

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ABclonal:Western blot - Phospho-MEK1-S298 Rabbit mAb (AP1020)}

Western blot - Phospho-MEK1-S298 Rabbit mAb (AP1020)

Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (AP1020) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

* For research use only. Not for therapeutic or diagnostic purposes.

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