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Phospho-MEK1-T292 Rabbit mAb (AP1021)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb (AP1021) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot analysis of lysates from HeLa cells, using Phospho-MEK1-T292 Rabbit mAb (A12687). HeLa cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product name	Phospho-MEK1-T292 Rabbit mAb
Catalog No.	AP1021
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1566

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T292 of human Phospho-MEK1-T292 (Q02750).
Sequence	PRTPG
Gene ID
Swiss Prot
Synonyms	MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1; Phospho-MEK1-T292
Calculated MW	43kDa
Observed MW	45kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C6 treated by Nocodazole, HeLa treated by Nocodazole
Cellular location	Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Customer validation	WB(Bos taurus, Mus musculus, Rattus norvegicus, Gallus gallus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-MEK1-T292 Rabbit mAb images

Western blot - Phospho-MEK1-T292 Rabbit mAb (AP1021)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-MEK1-T292 Rabbit mAb (AP1021)

Product:

Phospho-MEK1-T292 Rabbit mAb

Journal:

Journal of reproductive immunology

Application:

IF:

2.9

Species:

Bos taurus

PMID:

36252394

Title:

Enhanced expression of miR-26a ameliorates lipopolysaccharide-induced endometritis by targeting MAP3K8 to inactivate MAPK signaling pathway

References for Phospho-MEK1-T292 Rabbit mAb (AP1021)

Product:

Phospho-MEK1-T292 Rabbit mAb

Journal:

Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology

Application:

IF:

Species:

Mus musculus

PMID:

36592742

Title:

NeiyiKangfu tablets control the progression of endometriosis through inhibiting RAF/MEK/ERK signal pathway by targeting RKIP

References for Phospho-MEK1-T292 Rabbit mAb (AP1021)

Product:

Phospho-MEK1-T292 Rabbit mAb

Journal:

Neural plasticity

Application:

IF:

3.1

Species:

Rattus norvegicus

PMID:

36910013

Title:

Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation - PMC

References for Phospho-MEK1-T292 Rabbit mAb (AP1021)

Product:

Phospho-MEK1-T292 Rabbit mAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

36621100

Title:

Ligusticum chuanxiong promotes the angiogenesis of preovulatory follicles (F1-F3) in late-phase laying hens

References for Phospho-MEK1-T292 Rabbit mAb (AP1021)

Product:

Phospho-MEK1-T292 Rabbit mAb

Journal:

Application:

IF:

Species:

Rattus norvegicus

PMID:

38726683

Title:

The in vitro mechanism of Vaspinregulates the proliferation and steroidogenesis of rat lutein cells

References for Phospho-MEK1-T292 Rabbit mAb (AP1021)

Product:

Phospho-MEK1-T292 Rabbit mAb

Journal:

Materials today. Bio

Application:

IF:

8.7

Species:

Mus musculus

PMID:

39328787

Title:

Multifunctional vitamin D-incorporated PLGA scaffold with BMP/VEGF-overexpressed tonsil-derived MSC via CRISPR/Cas9 for bone tissue regeneration - PMC

References for Phospho-MEK1-T292 Rabbit mAb (AP1021)

Product:

Phospho-MEK1-T292 Rabbit mAb

Journal:

Frontiers in microbiology

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

39360313